Size:50 ug
$185.00
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OAED00168
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Anti-HSP25 Antibody (OAED00168)

Catalog#: OAED00168
Domestic: within 1 week delivery | International: within 1 week
More Information
Predicted Species Reactivity Human, Mouse, Rat, Bovine, Dog, Gerbil, Guinea Pig, Hamster, Pig
Clonality Polyclonal
Host Rabbit
Application EM, ICC, IF, IHC, IP, WB
Reconstitution and Storage Long Term Storage : -20C
Replacement Item This antibody may replace item sc-1048 from Santa Cruz Biotechnology.
Immunogen Recombinant mouse Hsp25.
Datasheets/Manuals Printable datasheet for Anti-HSP25 Antibody (OAED00168)OAED00168
Specificity Recognizes human, mouse, rat, bovine, dog, gerbil, guinea pig, hamster, and pig HSP25. Detects a band of ~25kDa by Western blot.
Formulation Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Purity Protein A affinity purified.
Application Info Electron Microscopy
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation (1:100)
Western Blot (1:1000, colorimetric)
Optimal conditions must be determined individually for each application.
Target Reference A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice: D. Jaarsma, et al. ; Hum. Mol. Genet. 17, 2849 (2008), Application(s): IF using mouse tissue, Abstract;
Autoantibodies in the Autoimmune Disease Pemphigus Foliaceus Induce Blistering via p38 Mitogen-Activated Protein Kinase-Dependent Signaling in the Skin: D. Rubenstein, et al. ; Am. J. Pathol. 173, 1628 (2008), Application(s): WB using human tissue, Abstract;
Cell dynamics in the pulpal healing process following cavity preparation in rat molars: H. Ohshima, et al. ; Histochem. Cell Biol. 130, 773 (2008), Application(s): IHC, EM using rat tissue, Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: I. Brown, et al. ; Cell Stress Chaperones 12, 51 (2007), Application(s): WB, IF using rat tissue, Abstract;
Gender differences in the expression of heat shock proteins: the effect of estrogen: A.A. Knowlton, et al. ; Am. J. Physiol. Heart Circ. Physiol. 285, H687 (2003), Application(s): WB using rat samples, Abstract;
Hsp27 associates with actin and limits injury in energy depleted renal epithelia: N.J. Siegel, et al. ; J. Am. Soc. Nephrol. 13, 98 (2003), Application(s): WB using porcine samples, Abstract;
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons: F.R. Sharp, et al. ; J. Cereb. Blood Flow Metab. 22, 183 (2002), Application(s): IHC using rat & gerbil samples, Abstract;
Stress-mediated signaling in PC12 cells - the role of the small heat shock protein, Hsp27, and Akt in protecting cells from heat stress and nerve growth factor withdrawal: C. Yegappan, et al. ; J. Neurochem. 83, 452 (2002), Application(s): IP using human samples, Abstract;
Crosstalk between p38, Hsp25 and Akt in spinal motor neurons after sciatic nerve injury: D.J. Wolgemuth, et al. ; Brain Res. Mol. Brain Res. 93, 199 (2001), Application(s): ICC, IP, WB, IHC using mouse samples, Abstract;
Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress: B.E. Sumpio, et al. ; Am. J. Physiol. Heart Circ. Physiol. 280, H189 (2001), Application(s): WB using bovine samples, Abstract;
Gene Symbol HSPB1
Alias Symbols CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102,
NCBI Gene Id 3315
Protein Name Heat shock protein beta-1
Description of Target Hsp25 is a member of the family of small heat shock proteins (sHsps), an evolutionarily conserved family of stress proteins which exhibit moleclular weights between 14-45 kDa. The sHsps are related to the alpha-crystallins, and generally exist as large oligomeric complexes that serve to stabilize and assist in refolding of denatured proteins. Hsp25 is highly homologous to the human Hsp27, and its expression is seen in many species, including mouse, rat, bovine, canine, and hamster cells.
Swissprot Id P04792
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express HSPB1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express HSPB1.
  1. What is the species homology for "Anti-HSP25 Antibody (OAED00168)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Dog, Gerbil, Guinea Pig, Hamster, Pig".

  2. How long will it take to receive "Anti-HSP25 Antibody (OAED00168)"?

    This item is available "Domestic: within 1 week delivery | International: within 1 week".

  3. What buffer format is "Anti-HSP25 Antibody (OAED00168)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Anti-HSP25 Antibody (OAED00168)"?

    This target may also be called "CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, " in publications.

  5. What is the shipping cost for "Anti-HSP25 Antibody (OAED00168)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Anti-HSP25 Antibody (OAED00168)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Anti-HSP25 Antibody (OAED00168)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Anti-HSP25 Antibody (OAED00168)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "HSPB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "HSPB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "HSPB1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "HSPB1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "HSPB1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "HSPB1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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