Anti-HO-1 (HO-1-1) Antibody (OAED00115)

Catalog No: OAED00115

Size:50 ug

Western blot analysis of HO-1 (Hsp32), mAb (HO-1-1): Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.

Immunohistochemistry analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10 ug/ml.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Clonality: Monoclonal
• Clone: HO-1-1
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunocytochemistry|Western blot
• Reconstitution and Storage: Long Term Storage : -20C
  
• Immunogen: Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.
• Specificity: Recognizes human, mouse, rat, bovine and dog HO-1. Detects a band of ~32kDa by Western blot.
• Formulation: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Purity: Protein G affinity purified.
  
• Application Info: Flow Cytometry (10μg/ml)
  Immunocytochemistry
  Immunohistochemistry (paraffin sections)
  Western Blot (1:1000 ECL)
  Optimal conditions must be determined individually for each application.
• Reference: Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress: F.A. Arosa, et al. ; Free Radic. Biol. Med. 35, 1404 (2003), Application(s): WB using human samples, Abstract;
  Defective hematopoiesis and hepatic steatosis in mice with combined deficiencies of the genes encoding Fancc and Cu/Zn superoxide dismutase: F.R. Jirik, et al. ; Blood 98, 1003 (2001), Application(s): WB using mouse samples, Abstract;
  Hmox-1 constitutes an adaptive response to effect antioxidant cardioprotection: a study with transgenic mice heterozygous for targeted disruption of the Heme oxygenase-1 gene: D.K. Das, et al. ; Circulation 103, 1695 (2001), Application(s): WB using mouse samples, Abstract;
  

Target Info

• Gene Symbol: HMOX1
• Gene Full Name: heme oxygenase 1
• Alias Symbols: bK286B10;heat shock protein, 32-kD;heme oxygenase (decycling) 1;heme oxygenase 1;HMOX1D;HO-1;HSP32.
• NCBI Gene Id: 3162
• Protein Name: Heme oxygenase 1
• Description of Target: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
• Uniprot ID: P09601