Anti-HO-1 Antibody (OAED00207)

Catalog No: OAED00207

Size:50 ug

Western blot analysis of HO-1 pAb: Lane 1: HO-1 Recombinant Protein; Lane 2: Human Liver Microsomes; Lane 3: Rat Liver Microsomes; Lane 4: Mouse Liver Microsomes; Lane 5: Canine Liver Microsomes.

Immunohistochemistry analysis of Alzheimer's diseased section stained with HO-1 (Hsp32) pAb.

Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10 ug/ml.

Crystal structure of human HO-1 (Hsp32) in complex with its substrate heme.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rabbit|Rat|Sheep
• Product Format: Liquid PBS containing 50% glycerol and 0.09% sodium azide
• Clonality: Polyclonal
• Host: Rabbit
• Application: Flow cytometry|Immunocytochemistry|Western blot
• Reconstitution and Storage: Store at -20°C. Avoid freeze/thaw cycles.
• Immunogen: Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region.
• Purification: Protein A affinity purified
• Concentration: 1 mg/ml
• Application Info: WB: 1:1000, ECL
• Reference: Adaptive HNE-Nrf2-HO-1 pathway against oxidative stress is associated with acute gastric mucosal lesions: T. Ueyama, et al. ; Am. J. Physiol. Gastrointest. Liver Physiol. 295, G460 (2008), Application(s): WB, IHC using rat cell lysates & tissue, Abstract;
  Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis. 52, 972 (2008), Application(s): IHC using human tissue, Abstract;
  Induction of Heme Oxygenase-1 Is a Beneficial Response in a Murine Model of Venous Thrombosis: K. Nath, et al. ; Am. J. Pathol. 173, 1882 (2008), Application(s): IHC using mouse tissue, Abstract;
  Comparison of the heme-free and -bound crystal structures of human heme oxygenase-1: L. Lad, et al.; J. Biol. Chem. 278, 78 (2003), (ref. 6), Abstract;
  Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress: F.A. Arosa, et al. ; Free Radic. Biol. Med. 35, 1404 (2003), Application(s): Flow using human samples, Abstract;
  Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress: A.M. Fonseca, et al.; Free Radic. Biol. Med. 35, 1404 (2003), (ref. 7), Abstract;
  Role of heme oxygenase-1 protein in the neuroprotective effects of cyclopentenone prostaglandin derivatives under oxidative stress: Y. Watanabe, et al. ; Eur. J. Neurosci. 17, 2249 (2003), Application(s): ICC, WB using mouse samples, Abstract;
  Role of heme oxygenase-1 protein in the neuroprotective effects of cyclopentenone prostaglandin derivatives under oxidative stress: T. Satoh, et al.; Eur. J. Neurosci. 17, 2249 (2003), (ref. 8), Abstract;
  Glucose deprivation induces heme oxygenase-1 gene expression by a pathway independent of the unfolded protein response: A. Agarwal, et al. ; J. Biol. Chem. 277, 1933 (2002), Application(s): WB using human samples, Abstract;
  Regulation and localization of HSP70 and HSP25 in the kidney of rats undergoing long-term administration of angiotensin II: N. Ishizaka, et al.; Hypertension 39, 122 (2002), (ref. 4), Abstract;
  Oxidative stress and induction of heme oxygenase-1 in the kidney in sickle cell disease: R.P. Hebbel, et al. ; Am. J. Pathol. 158, 893 (2001), Application(s): ICC using human & rat samples, Abstract;
  Induction of heme oxygenase-1 modulates cis-aconitase activity in lens epithelial cells: U.P. Andley, et al. ; Biochem. Biophys. Res. Commun. 270, 324 (2000), Application(s): WB using rabbit samples, Abstract;
  Induction of heme oxygenase-1 modulates cis-aconitase activity in lens epithelial cells: D.M. Rzymkiewicz, et al.; BBRC 270, 324 (2000), (ref. 9), Abstract;
  Heme oxygenase: recent advances in understanding its regulation and role: K.K. Elbirt & H.L. Bonkovsky; Proc. Assoc. Am. Physicians 111, 438 (1999), (ref. 1), Abstract;
  Peroxynitrite induces haem oxygenase-1 in vascular endothelial cells: a link to apoptosis: R. Foresti, et al.; Biochem J. 339 (Pt 3), 729 (1999), (ref. 3), Abstract;
  Expression pattern of heme oxygenase isoenzymes 1 and 2 in normal and stress-exposed rat liver: M. Bauer, et al. ; Hepatology 27, 829 (1998), Application(s): Flow using rat samples, Abstract;
  Carbon monoxide formation in the ductus arteriosus in the lamb: implications for the regulation of muscle tone: C. Ackerley, et al. ; Br. J. Pharmacol. 120, 599 (1997), Application(s): IHC using sheep samples, Abstract;
  Carbon monoxide formation in the ductus arteriosus in the lamb: implications for the regulation of muscle tone: F. Coceani, et al.; Br. J. Pharmacol. 120, 599 (1997), (ref. 10), Abstract;
  Expression of heme oxygenase-1 (HSP32) in human prostate: normal, hyperplastic, and tumor tissue distribution: M.D. Maines & P.A. Abrahamsson; Urology 47, 727 (1996), (ref. 2), Abstract;
  Heme oxygenase-1 is associated with the neurofibrillary pathology of Alzheimers disease: M.A. Smith, et al.; Am. J. Pathol. 145, 42 (1994), (ref. 5), Abstract;
  

Target Info

• Gene Symbol: Hmox1
• Gene Full Name: heme oxygenase 1
• Alias Symbols: heme oxygenase (decycling) 1;heme oxygenase (decyclizing) 1;heme oxygenase 1;Heox;HEOXG;Hmox;Ho1;Ho-1;hsp32.
• NCBI Gene Id: 24451
• Protein Name: Heme oxygenase 1
• Description of Target: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
• Uniprot ID: P06762
• Molecular Weight: ~32 kDa by WB