Anti-Granzyme B (human) (B18.1) Antibody (OAED00297)

Catalog No: OAED00297

Size:100 ug

Figure: Detection of granzyme B in a human CD8+ T cell clone. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20C for 5 min, then in acetone at -20C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 ug/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.

Schematic structure of human granzyme B (recombinant, aa. 7-227).

Figure: Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: B18.1
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: FC, ICC, IHC, IP, WB
• Reconstitution and Storage: Long Term Storage : +4C
  Handling : Do not freeze/thaw.
  Stable for at least 6 months after receipt when stored at +4C undiluted.
• Immunogen: Recombinant human granzyme B (aa 7-227).
• Concentration: 1mg/ml
• Specificity: Recognizes human granzyme B.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: ≥95% (SDS-PAGE).
• Application Info: Flow Cytometry
  Immunocytochemistry
  Immunohistochemistry (frozen sections, paraffin sections)
  Immunoprecipitation
  Western Blot
• Reference: Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells: R. Dressel, et al.; J. Biol. Chem. 279, 20200 (2004), Abstract; Full Text
  Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense: C. Berthou, et al.; J. Immunol. 159, 5293 (1997), Abstract;
  Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc. 28, 1964 (1996), Abstract;
  Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood 86, 3500 (1995), Abstract;
  Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol. 24, 229 (1994), Abstract;
  Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation 57, 322 (1994), Abstract;
  

Target Info

• Gene Symbol: GZMB
• Alias Symbols: C11, HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1
• NCBI Gene Id: 3002
• Protein Name: Granzyme B
• Uniprot ID: P10144