Anti-GluR2/3 (OAPC00142)

Catalog No: OAPC00142 (Formerly GWB-F53786)

Size:100 ul

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR2/3 protein.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Zebrafish
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C; stable for at least one year.
• Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of rat GluR2/3.
• Purification: Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat; Zebrafish
• Specificity: Specific for the ~100k GluR2/3 protein.
• Application Info: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with chicken, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
  Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR2/3 protein.
  
• Reference: Chung HJ, Steinberg JP, Huganir RL, Linden DJ (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755.
  Duprat F, Daw M, Lim W, Collingridge G, Isaac J (2003) GluR2 protein-protein interactions and the regulation of AMPA receptors during synaptic plasticity. Philos Trans R Soc Lond B Biol Sci 358:715-720.
  Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108.
  Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560.
  Seidenman KJ, Steinberg JP, Huganir R, Malinow R (2003) Glutamate receptor subunit 2 serine 880 phosphorylation modulates synaptic transmission and mediates plasticity in CA1 pyramidal cells. J Neurosci 23:9220-9228.
  Yan J, Zhang YF, Jia ZP, Taverna FA, McDonald RJ, Muller RU, Roder JC (2002) Place-cell impairment in glutamate receptor 2 mutant mice. J Neurosci 22:NIL7-NIL11.

Target Info

• Gene Symbol: GRIA2/3
• Alias Symbols: GluA2, GluR2, gluR-B, GluR-K2
• NCBI Gene Id: 29627
• Protein Name: Glutamate receptor 2
• Description of Target: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990;Hollmann and Heinemann, 1994). The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory (Duprat et al., 2003;Seidenman et al., 2003;Chung et al., 2003;Yan et al., 2002).
• Uniprot ID: P19491
• Protein Accession #: NP_001077280.1
• Molecular Weight: ~100kDa