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Anti-Glial Fibrillary Acidic Protein (GFAP) (OAPC00115)

100 ul
$269.00
In Stock
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Predicted Species Reactivity:
Human, Mouse, Rat
Product Format:
Liquid. 10mM sodium azide.
Clonality:
Polyclonal
Host:
Chicken
Application:
WB, IF
Reconstitution and Storage:
Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Gene Symbol:
GFAP
NCBI Gene Id:
281189
Protein Name:
Glial fibrillary acidic protein
Swissprot Id:
Q28115
Protein Accession #:
NP_776490.2
Replacement Item:
This antibody may replace item sc-115582 from Santa Cruz Biotechnology.
Description of Target:
Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).
Molecular Weight:
~50kDa
Purification:
Total IgY fraction.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GFAP.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express GFAP.
Immunogen:
Recombinant and purified bovine GFAP.
Predicted Homology Based on Immunogen Sequence:
Human; Mouse; Rat
Datasheets/Manuals:
Printable datasheet for OAPC00115
Specificity:
Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.
Application Info:
The antibody has been directly tested for reactivity in human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.
Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
Target Reference:
1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972).
2. Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001)

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