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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Anti-GFP Polyclonal Antibody (OAAI00693) |
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Predicted Species Reactivity | GFP Tag |
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Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. |
:: | Positive Control: Recombinant or native GFP |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A full length GFP recombinant protein. |
Purification | Affinity Purified |
Concentration | 100ug/200ul |
Specificity | GFP |
Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Reference | Prendergast F, Mann K (1978). "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea". Biochemistry 17 (17): 3448–53. |
Gene Symbol | gfp |
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Gene Full Name | green fluorescent protein |
Alias Symbols | green fluorescent protein;pCmGFP_001. |
NCBI Gene Id | 7011691 |
Description of Target | The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substRates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. |
Molecular Weight | 26 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-GFP Polyclonal Antibody (OAAI00693)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "GFP Tag".
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How long will it take to receive "Anti-GFP Polyclonal Antibody (OAAI00693)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-GFP Polyclonal Antibody (OAAI00693)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-GFP Polyclonal Antibody (OAAI00693)"?
This target may also be called "green fluorescent protein;pCmGFP_001." in publications.
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What is the shipping cost for "Anti-GFP Polyclonal Antibody (OAAI00693)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-GFP Polyclonal Antibody (OAAI00693)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-GFP Polyclonal Antibody (OAAI00693)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "26".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-GFP Polyclonal Antibody (OAAI00693)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "gfp"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "gfp"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "gfp"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "gfp"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "gfp"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "gfp"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.