Anti-GAP-43 (OAPC00136)

Catalog No: OAPC00136

Size:100 ul

Western blot of rat cortex lysate showing specific immunolabeling of the ~ 43k GAP43 protein.

Immunochemical staining of mixed neuron/glial cultures showing GAP43 (green) labeling of numerous axonal and dendritic profiles and anti-alpha II spectrin (red) (cat. # OAPC00144).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Total IgY fraction in PBS + 10mM NaN₃. Adequate amount of material to conduct 10-mini Western blots
• Clonality: Polyclonal
• Host: Chicken
• Application: WB, IF
• Reconstitution and Storage: For long term storage -20C is recommended. Stable at -20C for at least 1 year.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region of the rat GAP43 sequence.
• Purification: Total IgY fraction.
• Concentration: 15 mg/ml
• Specificity: Specific for the ~43k GAP-43 protein. The antibody has been directly tested for reactivity in Western blots with human, mouse, and rat tissue.
• Application Info: WB: 1:2000
  IF: 1:500
• Reference: Benowitz LI, Routtenberg A (1997) Gap-43: An intrinsic determinant of neuronal development and plasticit. Trends Neurosci 20:84-91.
  He, Q, Dent, EW, Meiri, KF (1997) Modulation of actin filament behavior by Gap-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17:3515-3524.
  Hulo S, Alberi, S, Laux T, Muller D, Caroni P (2002) A point mutant of Gap-43 induces enhanced short-term and long-term hippocampal plasticity. Eur J Neurosci 15:1976-1982.
  Strittmatter SM, Fankhauser C, Huang PL, Mashimo H, Fishman MC (1995) Neuronal path finding is abnormal in mice lacking the neuronal growth cone protein Gap-43," Cell 80:445-452.
  Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 - 14444.

Target Info

• Gene Symbol: GAP43
• Alias Symbols: Basp2
• NCBI Gene Id: 29423
• Protein Name: Neuromodulin
• Description of Target: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
• Uniprot ID: P07936
• Protein Accession #: NP_058891.1
• Molecular Weight: ~43kDa