Size:100 ul
$309.00
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SKU
OAPC00118
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GABRA1 Antibody (OAPC00118)

Catalog#: OAPC00118
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-6D8090
More Information
Predicted Species Reactivity Cow, Dog, Human, Mouse, Rat
Product Format Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Clonality Polyclonal
Host Rabbit
Application WB
Additional Information Packaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and Storage For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Replacement Item This antibody may replace item sc-14005 from Santa Cruz Biotechnology.
Immunogen Fusion protein from the cytoplasmic loop of the alpha 1-subunit of rat GABAA receptor.
Purification Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Predicted Homology Based on Immunogen Sequence Cow; Dog; Human; Mouse; Rat
Datasheets/Manuals Printable datasheet for OAPC00118
Specificity Specific for the ~51k alpha 1-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 1-subunit knockout animals.
Application Info The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with bovine, canine, human, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Western blot of mouse forebrain lysates from wild type (Control) and alpha 1-knockout (alpha 1-K/O) animals showing specific immunolabeling of the ~51k alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.
Target Reference McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha 1-subtype. Nature Neurosci 3:587-592.
Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha 1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma 2-subunit. Biochem Pharmacol 68:1621-1629.
Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.
Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653
.Mridula Rewal, Rachel Jurd, T. Michael Gill, Dao-Yao He, Dorit Ron, and Patricia H. Janak (2009) alpha 4-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol J. Neurosci., 29: 543 - 549
Publications

Zhao, L., Sakagami, H. & Suzuki, T. Detergent-dependent separation of postsynaptic density, membrane rafts and other subsynaptic structures from the synaptic plasma membrane of rat forebrain. J. Neurochem. 131, 147-62 (2014). WB, Cow, Dog, Human, Mouse, Rat 24985044

Gene Symbol GABRA1
NCBI Gene Id 29705
Protein Name Gamma-aminobutyric acid receptor subunit alpha-1
Description of Target Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Swissprot Id P62813
Protein Accession # NP_899155.1
Molecular Weight ~51kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express GABRA1.
RNA Seq Find tissues and cell lines supported by DNA array analysis to express GABRA1.
  1. What is the species homology for "GABRA1 Antibody (OAPC00118)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Cow, Dog, Human, Mouse, Rat".

  2. How long will it take to receive "GABRA1 Antibody (OAPC00118)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "GABRA1 Antibody (OAPC00118)" provided in?

    This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "GABRA1 Antibody (OAPC00118)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "GABRA1 Antibody (OAPC00118)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "GABRA1 Antibody (OAPC00118)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "GABRA1 Antibody (OAPC00118)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "~51kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "GABRA1 Antibody (OAPC00118)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "GABRA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "GABRA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "GABRA1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "GABRA1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "GABRA1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "GABRA1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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