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GABRA1 Antibody (OAPC00118)

100 ul
$349.00
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Gene Symbol:
GABRA1
NCBI Gene Id:
29705
Protein Name:
Gamma-aminobutyric acid receptor subunit alpha-1
Swissprot Id:
P62813
Protein Accession #:
NP_899155.1
Replacement Item:
This antibody may replace item sc-14005 from Santa Cruz Biotechnology.
Description of Target:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Molecular Weight:
~51kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GABRA1.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express GABRA1.
Immunogen:
Fusion protein from the cytoplasmic loop of the alpha 1-subunit of rat GABAA receptor.
Predicted Species Reactivity:
Cow, Dog, Human, Mouse, Rat
Predicted Homology Based on Immunogen Sequence:
Cow; Dog; Human; Mouse; Rat
Product Format:
Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and Storage:
For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Datasheets/Manuals:
Printable datasheet for OAPC00118
Specificity:
Specific for the ~51k alpha 1-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 1-subunit knockout animals.
Application Info:
The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with bovine, canine, human, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Western blot of mouse forebrain lysates from wild type (Control) and alpha 1-knockout (alpha 1-K/O) animals showing specific immunolabeling of the ~51k alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.
Additional Information:
Packaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Target Reference:
McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha 1-subtype. Nature Neurosci 3:587-592.
Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha 1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma 2-subunit. Biochem Pharmacol 68:1621-1629.
Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.
Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653
.Mridula Rewal, Rachel Jurd, T. Michael Gill, Dao-Yao He, Dorit Ron, and Patricia H. Janak (2009) alpha 4-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol J. Neurosci., 29: 543 - 549
Publications:

Zhao, L., Sakagami, H. & Suzuki, T. Detergent-dependent separation of postsynaptic density, membrane rafts and other subsynaptic structures from the synaptic plasma membrane of rat forebrain. J. Neurochem. 131, 147-62 (2014). WB, Cow, Dog, Human, Mouse, Rat 24985044

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