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Datasheets/Manuals | Printable datasheet for Anti-eNOS Antibody (OAAI00234) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be enhanced through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. There are three types of NOS: two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II). iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. The phosphorylation of eNOS by Protein kinase C was associated with an increase in NO production. |
:: | Positive Control: placenta |
:: | Cellular Location: Cytoplasmic |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A synthetic peptide corresponding to the internal sequence (residing in 550-650aa) of human eNOS protein. |
Purification | Affinity Purified |
Concentration | 100ug/200ul |
Specificity | NOS3 |
Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Reference | Louis J. Ignarro, Functional interaction of endothelial nitric oxide synthase with a voltage-dependent anion channel. Proc Natl Acad Sci U S A. 99(20): 13108–13113, 2002. |
Gene Symbol | NOS3 |
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Gene Full Name | nitric oxide synthase 3 |
Alias Symbols | cNOS;constitutive NOS;ECNOS;EC-NOS;endothelial NOS;eNOS;nitric oxide synthase 3 (endothelial cell);nitric oxide synthase 3 transcript variant eNOS-delta20;nitric oxide synthase 3 transcript variant eNOS-delta20-21;nitric oxide synthase 3 transcript variant eNOS-delta21;nitric oxide synthase, endothelial;NOS type III;NOSIII. |
NCBI Gene Id | 4846 |
Description of Target | NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be enhanced throμgh cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. There are three types of NOS: two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II). iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. The phosphorylation of eNOS by Protein kinase C was associated with an increase in NO production. |
Uniprot ID | P51511 |
Molecular Weight | 140 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-eNOS Antibody (OAAI00234)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Anti-eNOS Antibody (OAAI00234)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-eNOS Antibody (OAAI00234)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-eNOS Antibody (OAAI00234)"?
This target may also be called "cNOS;constitutive NOS;ECNOS;EC-NOS;endothelial NOS;eNOS;nitric oxide synthase 3 (endothelial cell);nitric oxide synthase 3 transcript variant eNOS-delta20;nitric oxide synthase 3 transcript variant eNOS-delta20-21;nitric oxide synthase 3 transcript variant eNOS-delta21;nitric oxide synthase, endothelial;NOS type III;NOSIII." in publications.
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What is the shipping cost for "Anti-eNOS Antibody (OAAI00234)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-eNOS Antibody (OAAI00234)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-eNOS Antibody (OAAI00234)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "140".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-eNOS Antibody (OAAI00234)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NOS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NOS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NOS3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NOS3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NOS3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NOS3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.