Anti-Doublecortin (DCX) (OAPC00106)

Catalog No: OAPC00106

Size:100 ul

Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 & 45k doublecortin protein.

Immunofluorescence of cultured rat neurons showing strong cytoplasmic staining of doublecortin (green) in developing neurons and GFAP (cat. # OAPC00115) in red.

Product Info

• Product Format: Liquid PBS + 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western blots
• Clonality: Monoclonal
• Clone: 3E1
• Isotype: IgG2a
• Host: Mouse
• Application: WB, IF
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
• Immunogen: Full length recombinant human doublecortin expressed in and purified from E. coli.
• Purification: Affinity purified from tissue culture supernatant.
• Concentration: 1 mg/ml
• Specificity: Specific for the ~35 & 45 k doublecortin protein.
• Application Info: WB: 1:5000
  IF: 1:1000
• Reference: Brown JP, Couillard-Despres S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (2003) Transient expression of doublecortin during adult neurogenesis. J Comp Neurol. Dec 1;467(1):1-10.
  des Portes V, Pinard JM, Billuart P, Vinet MC, Koulakoff A, Carri&© A, Gelot A, Dupuis E, Motte J, Berwald-Netter Y, Catala M, Kahn A, Beldjord C and Chelly J. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell 92:51-61.
  Gleeson JG, Allen KM, Fox JW, Lamperti ED, Berkovic S, Scheffer I, Cooper EC, Dobyns WB, Minnerath SR, Ross ME and Walsh CA. (1998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell 92:63-72.

Target Info

• Gene Symbol: DCX
• Gene Full Name: Doublecortin
• Alias Symbols: DC, DBCN, LISX, SCLH, XLIS
• NCBI Gene Id: 1641
• Protein Name: Neuronal migration protein doublecortin
• Description of Target: Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to
  X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998).
• Uniprot ID: O43602
• Protein Accession #: NP_000546.2