Anti-DnaJ (E. coli) Antibody (OAED00145)

Catalog No: OAED00145

Size:50 ug

Western blot analysis: Lane 1: DnaJ Recombinant E. coli Protein.

Product Info

• Predicted Species Reactivity: Escherichia coli
• Product Format: Liquid. PBS containing 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Application: Immunoprecipitation|Western blot
• Reconstitution and Storage: Ships with blue ice
  Long Term Storage: -20°C
  Avoid freeze/thaw cycles.
• Immunogen: E. coli DnaJ.
• Purification: Protein A affinity purified
• Concentration: 1 mg/ml
• Application Info: Immunoprecipitation: 1:200
  Western Blot : 1:1000, ECL
  Optimal conditions must be determined individually for each application.
  Note: Detects a band of ~41 kDa Western Blot.

Target Info

• Gene Symbol: dnaJ
• Gene Full Name: chaperone protein DnaJ
• Alias Symbols: b0015;chaperone protein DnaJ;ECK0015;groP;grpC;Heat shock protein J;HSP40.
• NCBI Gene Id: 944753
• Protein Name: Chaperone protein DnaJ
• Description of Target: Interacts with DnaK and GrpE to disassemble a protein complex at the origins of replication of phage lambda and several plasmids. Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
• Uniprot ID: P08622
• Molecular Weight: ~41kDa