Anti-DARPP-32 (OAPC00098)

Catalog No: OAPC00098 (Formerly GWB-369615)

Size:100 ul

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.

Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C; stable for at least one year.
• Immunogen: Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
• Purification: Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
• Predicted Homology Based on Immunogen Sequence: Cow; Dog; Human; Mouse; Rat
• Specificity: Specific for the ~32k DARPP-32 protein.
• Application Info: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
  Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.
  Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.
• Reference: Bibb JA, Snyder GL, Nishi A, Yan Z, Meijer L, Fienberg AA, Tsai LH, Kwon YT, Girault JA, Czernik AJ, Huganir RL, Hemmings HC, Jr., Nairn AC, Greengard P (1999) Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons. Nature (London) 402:669-671.
  Fienberg, A.A., Hiroi, N., Mermelstein, P.G., Song, W., Snyder, G.L., Nishi, A., Cheramy, A. O'Callaghan, J.P., Miller, D.B., Cole, D.G., Corbett, R., Haile, C.N., Cooper, D,C., Onn, S.P., Grace, A.A., Ouimet, C.C., White, F.G., Hyman, S.E., Surmeier, D.G., Girault, J., Nestler, E.J. and Greengard, P. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842.
  Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G (2002) Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418:774-778.
  Maldve RE, Zhang TA, Ferrani-Kile K, Schreiber SS, Lippmann MJ, Snyder GL, Feinberg AA, Leslie SW, Gonzales RA, Morrisett RA (2002) DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nature Neurosci 5:641-648.
  Svenningsson P, Tzavara ET, Witkin JM, Fienberg AA, Nomikos GG, Greengard P (2002) Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac). Proc Natl Acad Sci USA 99:3182-3187.
  Michelle Niculescu, Shane A. Perrine, Jonathan S. Miller, Michelle E. Ehrlich, and Ellen M. Unterwald (2008) Trk: A Neuromodulator of Age-Specific Behavioral and Neurochemical Responses to Cocaine in Mice. J. Neurosci., Jan 2008; 28: 1198 - 1207.

Target Info

• Gene Symbol: PPP1R1B
• Alias Symbols: Darpp32, Darpp-32
• NCBI Gene Id: 360616
• Protein Name: Protein phosphatase 1 regulatory subunit 1B
• Description of Target: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
• Uniprot ID: Q6J4I0
• Protein Accession #: NP_612530.1
• Molecular Weight: ~32kDa