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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for OAPC00093 |
|---|
| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. 100 ul in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Monoclonal |
| Clone | F7E4 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB |
| Additional Information | Packaging : 100 ul in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | Store at -20C; stable for at least one year. |
| Immunogen | Recombinant full length human COBRA1 purified from E. coli |
| Purification | Protein G purified culture supernatant. |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
| Specificity | Specific for the ~62k COBRA1 protein in Western blots of neonatal rat brain lysate. |
| Application Info | The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue. Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 62K COBRA1 protein. |
| Reference | Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134-46. Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun.325 (2): 568-73. |
| Gene Symbol | COBRA1 |
|---|---|
| Alias Symbols | COBRA1, NELF-B |
| NCBI Gene Id | 25920 |
| Protein Name | Negative elongation factor B |
| Description of Target | COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ER alpha) and repress ER alpha -mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004) |
| Uniprot ID | Q8WX92 |
| Protein Accession # | NP_056271 |
| Nucleotide Accession # | NM_015456 |
| Molecular Weight | ~62kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-COBRA 1 (OAPC00093)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "Anti-COBRA 1 (OAPC00093)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-COBRA 1 (OAPC00093)" provided in?
This item is provided in "Liquid. 100 ul in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-COBRA 1 (OAPC00093)"?
This target may also be called "COBRA1, NELF-B" in publications.
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What is the shipping cost for "Anti-COBRA 1 (OAPC00093)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-COBRA 1 (OAPC00093)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-COBRA 1 (OAPC00093)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~62kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-COBRA 1 (OAPC00093)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "COBRA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "COBRA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "COBRA1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "COBRA1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "COBRA1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "COBRA1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
