Anti-Cit-Histone H3 Antibody (2/8/17) (OAAI00794)

Catalog No: OAAI00794

Size:100UG

Fluorescent Immuno Histochemistry: Serum-depleted 3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/ml Rb Anti-Cit-l Histone H3 (2/8/17) antibody, (Cat#OAAI00794), followed by then Rhodamine labeled Gt anti Rb IgG for visualization under fluorescent microscope

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K27. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
  Positive Control: 3T3 Nuclear Extract
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide containing N-terminus of human histone H3 with citrulline modification at 2/8/17.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: H3C1
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1γ Are Associated with Transcription Elongation throμgh Mammalian Chromatin . Molecular Cell (2003), Volume 19 , Issue 3 , Pages 381 - 391.
  David J Seward, et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nature Structural & Molecular Biology 14, 240 - 242 (2007)

Target Info

• Gene Symbol: H3C1
• Gene Full Name: H3 clustered histone 1
• Alias Symbols: H3 histone family, member A;H3/A;H3C10;H3C11;H3C12;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;HIST1H3A;histone 1, H3a;histone cluster 1 H3 family member a;histone cluster 1, H3a;histone H3.1;histone H3/a.
• NCBI Gene Id: 8350
• Description of Target: Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This Antibody is specific for histone H3 tri-methylated at K27. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The Antibody will react with any of the above species where the trimethylation modification is present.
• Uniprot ID: P68431
• Molecular Weight: 17