Anti-Cdk8 Polyclonal Antibody (OAAI00300)

Catalog No: OAAI00300

Size:100UG

WB: The cell lysate derived from HT29 was resolved on to 10% SDS- PAGE, transferred to NC membrane and immunoprobed by the Rabbit anti Cdk8 antibody, (Cat#OAAI00300 ) at 1:500 for 30 min @ RT. An immune- reactive band is observed around 54 kDa.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: A family of proteins designated as cdks belongs to the protein kinase superfamily. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. CDK8 kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
  Positive Control: Human lymphoma
  Cellular Location: Nuclear/Cytoplasmic
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide derived from C-terminus of human cdk8 protein. This sequence is identical to human, mouse and rat.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: CDK8
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested

Target Info

• Gene Symbol: CDK8
• Gene Full Name: cyclin dependent kinase 8
• Alias Symbols: CDK8 protein kinase;cell division protein kinase 8;cyclin-dependent kinase 8;IDDHBA;K35;mediator complex subunit CDK8;mediator of RNA polymerase II transcription subunit CDK8;protein kinase K35.
• NCBI Gene Id: 1024
• Description of Target: A family of proteins designated as cdks belongs to the protein kinase superfamily. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.
  Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. CDK8 kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
  
• Uniprot ID: P49336
• Molecular Weight: 54