Anti-CD29/INTEGRIN BETA 1 Antibody (OAAI00114)

Catalog No: OAAI00114

Size:100UG

WB: The cell lysate derived from Raji cells was immunoblotted by the Rabbit anti CD29 (Cat#OAAI00114) @ 1:500. An immunoreactive band around ~130 kDa was observed.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
  Positive Control: Raji cells. Tonsil
  Cellular Location: Cell membrane.
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: Recombinant protein encoding aa 579-799 of human CD29 expressed in E.Coli.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: ITGB1
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Chang,M.H., Lee,K., Lee,K.Y., Kim,Y.S., Kim,Y.K. and Kang,J.H. Prognostic role of integrin beta1, E-cadherin, and rac1 expression in small cell lung cancer. APMIS 120 (1), 28-38 (2012).
  

Target Info

• Gene Symbol: ITGB1
• Gene Full Name: integrin subunit beta 1
• Alias Symbols: CD29;FNRB;glycoprotein IIa;GPIIA;integrin beta 1;integrin beta-1;integrin VLA-4 beta subunit;integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12);MDF2;MSK12;very late activation protein, beta polypeptide;VLAB;VLA-BETA.
• NCBI Gene Id: 3688
• Description of Target: Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
  
• Uniprot ID: P05556
• Molecular Weight: 130