Anti-Cardif (human) (AT107) Antibody (OAED00260)

Catalog No: OAED00260

Size:100UG

Western blot analysis of Cardif (human), pAb (AT107): Lane 1: MW marker; Lane 2: HepG2; Lane 3: PALA; and Lane 4: HeLa. Additional bands observed probably represent isoforms or cleaved products of Cardif.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS containing 0.02% sodium azide
• Clonality: Polyclonal
• Host: Rabbit
• Application: Immunocytochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: Store short-term at 4°C; long-term at -20°C. Avoid freeze/thaw cycles. Stable for at least 1 year after receipt when stored at -20°C.
• Immunogen: Recombinant human Cardif (CARD adapter inducing interferon-beta) (aa 160-450).
• Purification: Protein A -- affinity purified
• Concentration: 1 mg/ml
• Specificity: Recognizes human cardif. Detects a band of ~65kDa by Western blot.
• Application Info: ICC: 1:500
  IP: 1:100
  WB: 1:2000
• Reference: Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system: P. Bellecave, et al.; Hepatology 51, 1127 (2010), Abstract;
  Cleavage of the IPS-1/Cardif/MAVS/VISA does not inhibit T cell-mediated elimination of hepatitis C virus non-structural 3/4A-expressing hepatocytes: G. Ahlen, et al.; Gut 58, 560 (2009), Abstract;
  Antiviral suppression vs restoration of RIG-I signaling by hepatitis C protease and polymerase inhibitors: Y. Liang, et al.; Gastroenterology 135, 1710 (2008), Abstract;
  Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008), Abstract;
  The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;
  Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006), Abstract;
  CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006), Abstract;
  Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005), Abstract;
  Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005), Abstract;
  IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005), Abstract;
  VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005), Abstract;

Target Info

• Gene Symbol: MAVS
• Gene Full Name: mitochondrial antiviral signaling protein
• Alias Symbols: CARD adapter inducing interferon beta;CARD adaptor inducing IFN-beta;CARDIF;IFN-B promoter stimulator 1;interferon beta promoter stimulator protein 1;IPS1;IPS-1;mitochondrial antiviral-signaling protein;putative NF-kappa-B-activating protein 031N;virus-induced signaling adaptor;virus-induced-signaling adapter;VISA.
• NCBI Gene Id: 57506
• Protein Name: Mitochondrial antiviral-signaling protein
• Description of Target: Required for innate immune defense against viruses. Acts downstream of DHX33, DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
• Uniprot ID: Q7Z434