Calretinin Antibody (OAPC00087)

Catalog No: OAPC00087

Size:100 ul

Western blot of rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.

Immunostaining of rat cerebellum showing fluorescence of calretinin containing cells.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 100 uL antibody per vial in 10mM Tris, 100mM NaCl. Adequate amount of material to conduct 10-mini Western blots
• Clonality: Polyclonal
• Host: Sheep
• Application: WB, IHC
• Reconstitution and Storage: Store at -20°C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
• Immunogen: Purified guinea pig calretinin.
• Purification: Protein A purified sheep serum.
• Concentration: 0.25 mg/mL
• Specificity: Specific for the ~29 kDa calretinin protein in Western blots of rat cerebellum. The antibody also works well for Immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.
• Application Info: The antibody has been directly tested for reactivity in rat. It is expected that this antibody will also react with human, mouse and non-human primates.
  Suggested dilutions are:
  WB ~ 1:1000
  IHC: ~ 1:500
• Reference: Attanoos RL, Dojcinov SD, Webb R, Gibbs AR. (2000) Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology.37(3):224-31.
  Isaacs KR, Winsky L, Strauss KI, Jacobowitz DM. (1995) Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell Tissue Res., 280(3):639-51.
  Lukas W, Jones KA (1994) Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neurosci. 61(2):307-16.
  Weisenhorn DMV, Weruaga-Prieto E, Celio MR (1996) Calretinin immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Exp. Brain Res. 108(1):101-112.

Target Info

• Gene Symbol: CALB2
• NCBI Gene Id: 117059
• Protein Name: Calretinin
• Description of Target: Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).
• Uniprot ID: P47728
• Protein Accession #: NP_446440.1
• Molecular Weight: ~29kDa