Anti-Beta III Tubulin (OAPC00075)

Catalog No: OAPC00075

Size:100 ul

Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.

Immunostaining of mouse dentate gyrus showing beta III tubulin in red and nuclei in blue. Photo courtesy of Robert Wine.

Product Info

• Predicted Species Reactivity: Most mammals
• Product Format: Liquid 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Clone: AA10
• Isotype: IgG
• Host: Mouse
• Application: WB, IHC
• Reconstitution and Storage: For long-term storage, -80C is recommended, but shorter-term storage at -20C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20C.
• Immunogen: Synthetic peptide corresponding to beta III tubulin.
• Purification: Protein G purified culture supernatant.
• Concentration: 1 mg/ml
• Specificity: Specific for the ~55kDa beta III tubulin protein in Western blots of rat brain extracts.
• Application Info: WB: 10,000
  IHC: 1:1000
• Reference: Lee MK, Rebhun LI, Frankfurter A (1990) Posttranslational modification of class III beta tubulin. Proc Natl Acad Sci USA 87(18): 7195-7199.
  Katsetos CD, Herman MM, Mork SJ (2003) Class III beta tubulin in human development and cancer. Cell Motil Cytoskeleton 55(2):77-96.

Target Info

• Gene Symbol: TUBB3
• NCBI Gene Id: 246118
• Protein Name: Tubulin beta-3 chain
• Description of Target: Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)
• Uniprot ID: Q4QRB4
• Protein Accession #: NP_640347.2
• Molecular Weight: ~55kDa