Anti-Bcl-rambo (human) (CT) (Rocky-2) Antibody (OAED00357)

Catalog No: OAED00357

Size:100UG

Figure: Immunofluorescence microscopy showing the detection of endogenous Bcl-rambo in HELA cells with the MAb to Bcl-rambo (human) (CT) (Rocky-2). Method: Following fixation with 2% paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS containing 0.02% sodium azide
• Clonality: Monoclonal
• Clone: Rocky-2
• Isotype: IgG1
• Host: Mouse
• Application: Immunocytochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: Short term storage store at 4°C, long term -20°C. Avoid freeze/thaw cycles. Stable for at least 1 year when stored at -20°C.
• Immunogen: Recombinant human Bcl-rambo.
• Concentration: 1 mg/ml
• Specificity: Recognizes the C-terminus of human Bcl-rambo (aa 224-459). Detects endogenous protein by ICC, IP and WB.
• Source: Purified from concentrated hybridoma tissue culture supernatant.
• Application Info: ICC: 1:300
  IP: 1:100
  WB: 1:1,000
  Optimal dilutions should be determined by the end user.
• Reference: Bcl-rambo beta, a special splicing variant with an insertion of an Alu-like cassette, promotes etoposide- and Taxol-induced cell death: P. Yi, et al.; FEBS Lett. 534, 61 (2003), Abstract; Full Text
  Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension: T. Kataoka, et al.; J. Biol. Chem. 276, 19548 (2001)

Target Info

• Gene Symbol: BCL2L13
• Gene Full Name: BCL2 like 13
• Alias Symbols: Bcl2-L-13;BCL2-like 13 (apoptosis facilitator);bcl-2-like protein 13;BCL-RAMBO;MIL1;Protein Mil1.
• NCBI Gene Id: 23786
• Protein Name: Bcl-2-like protein 13
• Description of Target: May promote the activation of caspase-3 and apoptosis.
• Uniprot ID: Q9BXK5