Anti-BAFF (Buffy-2) Antibody (OAED00305)

Catalog No: OAED00305

Size:100UG

Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: Buffy-2
• Isotype: IgM
• Host: Rat
• Application: Flow cytometry|Immunohistochemistry|Western blot
• Reconstitution and Storage: Long Term Storage : -20C
  Handling : Do not freeze/thaw.
  Stable for at least 6 months after receipt when stored as recommended.
• Immunogen: Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025).
• Concentration: 1mg/ml
• Specificity: Recognizes membrane-bound and soluble human and mouse (weak) BAFF.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: ≥95% (SDS-PAGE)
• Application Info: Flow Cytometry
  Immunohistochemistry (frozen sections, paraffin sections)
  Western Blot
• Reference: Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer 8, 76 (2008), Abstract; Full Text
  In vitro and in vivo activation induces BAFF and APRIL expression in B cells: V.T. Chu, et al.; J. Immunol. 179, 5947 (2007), Abstract;
  Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjogrens syndrome: M.V. Jonsson, et al.; J. Clin. Immunol. 25, 189 (2005), Abstract;
  Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitts lymphoma: C.A. Ogden, et al.; J. Immunol. 174, 3015 (2005), Abstract; Full Text
  BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004), Abstract;
  Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004), Abstract;
  Expression of BLyS and its Receptors in B-Cell Non-Hodgkin Lymphoma: Correlation with Disease Activity and Patient Outcome: A.J. Novak, et al.; Blood 104, 2247 (2004), Abstract;
  Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004), Abstract; Full Text
  Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogrens syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002), Abstract; Full Text
  Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice: S.D. Khare, et al.; PNAS 97, 3370 (2000), Abstract; Full Text
  BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999), Abstract; Full Text
  BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator: P.A. Moore, et al.; Science 285, 260 (1999), Abstract;
  Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kB, and c-Jun NH2-terminal kinase: A. Mukhopadhyay, et al.; J. Biol. Chem. 274, 15978 (1999), Abstract; Full Text
  Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations: F. Mackay, et al.; J. Exp. Med. 190, 1697 (1999), Abstract; Full Text
  TALL-1 is a novel member of the TNF family that is down-regulated by mitogens: H.B. Shu, et al.; J. Leukoc. Biol. 65, 680 (1999), Abstract;

Target Info

• Gene Symbol: TNFSF13B
• Gene Full Name: TNF superfamily member 13b
• Alias Symbols: ApoL related ligand TALL-1;B lymphocyte stimulator;BAFF;B-cell-activating factor;B-lymphocyte stimulator;BLYS;CD257;delta BAFF;Delta4 BAFF;dendritic cell-derived TNF-like molecule;DTL;epididymis secretory sperm binding protein;TALL1;TALL-1;THANK;TNF and ApoL-related leukocyte expressed ligand 1;TNF- and APOL-related leukocyte expressed ligand 1;TNF homolog that activates apoptosis;TNFSF20;TNLG7A;tumor necrosis factor (ligand) superfamily, member 13b;tumor necrosis factor (ligand) superfamily, member 20;tumor necrosis factor ligand 7A;tumor necrosis factor ligand superfamily member 13B;tumor necrosis factor superfamily member 13b;tumor necrosis factor-like protein ZTNF4;ZTNF4.
• NCBI Gene Id: 10673
• Protein Name: Tumor necrosis factor ligand superfamily member 13B
• Description of Target: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
• Uniprot ID: Q9Y275