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Catalog No: OAAI00694
Size:100UG
Price: $429.00
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OAAI00694
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Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)

Datasheets/ManualsPrintable datasheet for Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)
Product Info
Predicted Species ReactivityBovine|Chicken|Dog|Human|Invertebrate|Mouse|Rat
Product FormatLiquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
ClonalityPolyclonal
IsotypeRabbit IgG
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
Additional InformationBackground: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2).
::Positive Control: EGF-stimulated A431
::Cellular Location: Cytoplasm
Reconstitution and StorageThe antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
ImmunogenA synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha protein from human origin. It is identical among human, mouse, rat, chicken, bovine, dog, and insect species.
PurificationAffinity Purified
Concentration100ug/200ul
Target Post-Translational ModificationPhosphorylated
SpecificityPRKAA1
FormulationThis affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Application InfoWB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
ReferenceJing Chen et al. AMPK regulation of Mouse oocyte meiotic resumption in vitro. Developmental Biology, 291 (2), 227-238 (2006).
Gene SymbolPRKAA1
Gene Full Nameprotein kinase AMP-activated catalytic subunit alpha 1
Alias Symbols5'-AMP-activated protein kinase catalytic subunit alpha-1;5'-AMP-activated protein kinase, catalytic alpha-1 chain;ACACA kinase;acetyl-CoA carboxylase kinase;AMP -activate kinase alpha 1 subunit;AMP-activated protein kinase, catalytic, alpha-1;AMPK;AMPK alpha 1;AMPK subunit alpha-1;AMPK, alpha, 1;AMPKa1;HMGCR kinase;hydroxymethylglutaryl-CoA reductase kinase;protein kinase, AMP-activated, alpha 1 catalytic subunit;tau-protein kinase PRKAA1.
NCBI Gene Id5562
Description of TargetAMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and γ subunits.
Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit.
AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2).
Uniprot IDQ13131
Molecular Weight63
  1. What is the species homology for "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Chicken|Dog|Human|Invertebrate|Mouse|Rat".

  2. How long will it take to receive "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)" provided in?

    This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    This target may also be called "5'-AMP-activated protein kinase catalytic subunit alpha-1;5'-AMP-activated protein kinase, catalytic alpha-1 chain;ACACA kinase;acetyl-CoA carboxylase kinase;AMP -activate kinase alpha 1 subunit;AMP-activated protein kinase, catalytic, alpha-1;AMPK;AMPK alpha 1;AMPK subunit alpha-1;AMPK, alpha, 1;AMPKa1;HMGCR kinase;hydroxymethylglutaryl-CoA reductase kinase;protein kinase, AMP-activated, alpha 1 catalytic subunit;tau-protein kinase PRKAA1." in publications.

  5. What is the shipping cost for "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "63".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Anti-AMPK Alpha Antibody (Phospho-T 172) (OAAI00694)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "PRKAA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "PRKAA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "PRKAA1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "PRKAA1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "PRKAA1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "PRKAA1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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