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Datasheets/Manuals | Printable datasheet for OAPC00002 |
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Predicted Species Reactivity | Most mammals |
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Product Format | Liquid containing 10 mM sodium azide. |
Clonality | Monoclonal |
Clone | 1D2 |
Isotype | IgG1 |
Host | Mouse |
Application | WB, IF, IHC |
Additional Information | Preservative:10mM sodium azide. |
Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles. |
Immunogen | Recombinant rat alpha-internexin expressed in and purified from E. coli. |
Purification | Total IgG fraction |
Predicted Homology Based on Immunogen Sequence | Most mammals |
Specificity | Specific for the ~66k alpha Internexin protein. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections. |
Application Info | The antibody has been directly tested for reactivity in a wide variety of mammalian species. Immunostaining of cultured rat CNS cells showing specific labeling of alpha-internexin in neuronal processes in red and anti-chicken MAP2 antibody (cat. # OAPC00006) in green. Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein. Suggested dilutions are: WB:1:2000 IF:1:250 IHC:1:1000 |
Reference | 1. Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985). 2. McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002). 3. Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004). 4. Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004). 5. Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004). |
Gene Symbol | INA |
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Alias Symbols | Nf66, Inexa, Intlaa, alpha-Inx |
NCBI Gene Id | 24503 |
Protein Name | Alpha-internexin |
Description of Target | Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5). |
Uniprot ID | P23565 |
Protein Accession # | NP_062001.1 |
Molecular Weight | ~66kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Most mammals".
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How long will it take to receive "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Alpha-Internexin (NF66) (OAPC00002)" provided in?
This item is provided in "Liquid containing 10 mM sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
This target may also be called "Nf66, Inexa, Intlaa, alpha-Inx" in publications.
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What is the shipping cost for "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~66kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Alpha-Internexin (NF66) (OAPC00002)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "INA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "INA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "INA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "INA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "INA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "INA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.