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| Datasheets/Manuals | Printable datasheet for Anti-Actin Antibody Muscle Specific (OAAI00256) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Clonality | Monoclonal |
| Clone | HHF35 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Additional Information | Background: The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. It is involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. The levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading. |
| :: | Positive Control: Muscle |
| :: | Cellular Location: Cytoplasmic |
| Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
| Immunogen | A synthetic peptide corrsponding to the internal segment of human Actin |
| Purification | The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping |
| Concentration | 100ug/200ul |
| Specificity | AKTA1 |
| Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-Actin Antibody Muscle Specific (OAAI00256)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
This target may also be called "ACTA;actin, alpha skeletal muscle;ASMA;CFTD;CFTD1;CFTDM;MPFD;NEM1;NEM2;NEM3;nemaline myopathy type 3;SHPM." in publications.
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What is the shipping cost for "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "42".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Actin Antibody Muscle Specific (OAAI00256)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ACTA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ACTA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ACTA1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ACTA1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ACTA1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ACTA1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
