ANO1 ELISA Kit (Human) (OKEH08744)

Catalog No: OKEH08744

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ANO1 ELISA Kit (Human) (OKEH08744) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANO1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.6% (n = 20)
  Mean Inter-assay CV%: <=10.2% (n = 20)
• ELISA Kit Component:
  • ANO1 Microplate: 96 Wells (12 x 8 Well strips)
  • ANO1 Lyophilized Standard: 2
  • 100X Biotinylated ANO1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
  Subcellular Location: Cell membrane Multi-pass membrane protein Cytoplasm Cytoplasmic localization seen in neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors.
  Unigene: Hs.503074
  String: 9606.ENSP00000347454
  KEGG: hsa:55107
  MIM: 610108
  Pfam: PF16178
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.162ng/mL
• Specificity: Natural and recombinant Human Anoctamin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANO1
• Gene Full Name: anoctamin 1
• Alias Symbols: anoctamin 1, calcium activated chloride channel;anoctamin-1;Ca2+-activated Cl- channel;calcium activated chloride channel;discovered on gastrointestinal stromal tumors protein 1;DOG1;oral cancer overexpressed 2;Oral cancer overexpressed protein 2;ORAOV2;TAOS2;TMEM16A;Transmembrane protein 16A;transmembrane protein 16A (eight membrane-spanning domains);tumor-amplified and overexpressed sequence 2.
• NCBI Gene Id: 55107
• Protein Name: Q5XXA6
• Description of Target: Anoctamin-1
• Uniprot ID: https://www.uniprot.org/uniprot/Q5XXA6
• Protein Accession #: NP_060513.5
• Nucleotide Accession #: NM_018043.5