ANO1 Antibody - middle region (OAAB13882)

Catalog No: OAAB13882

Size:400ul

ANO1 Antibody (Center) (Cat. # OAAB13882) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ANO1 Antibody detected the ANO1 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Function: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
  Cellular Location: Cell membrane; Multi-pass membrane protein. Cytoplasm. Note=Cytoplasmic localization seen in neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors
  Tissue Location: Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ANO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the Central region of human ANO1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: ANO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
• Publications:

  Yin, L. et al. Glucose stimulates calcium-activated chloride secretion in small intestinal cells. Am. J. Physiol. Cell Physiol. 306, C687-96 (2014). 24477233

Target Info

• Gene Symbol: ANO1
• Alias Symbols: DOG1, TAOS2, ORAOV2, TMEM16A
• NCBI Gene Id: 55107
• Protein Name: Anoctamin-1
• Description of Target: ANO1 acts as a calcium-activated chloride channel. Required for normal tracheal development.
• Uniprot ID: Q5XXA6
• Protein Accession #: NP_060513.5
• Molecular Weight: 114078 Da