Annexin V : PE (OOTA00729)

Catalog No: OOTA00729

Mouse thymocytes were incubated overnight at 37C in medium containing 1 uM dexamethasone. Cells were harvested and stained with PE Annexin V (OOTA00729) and 7-AAD Staining Solution (OOTA00666).

Product Info

• Product Format: Liquid. 10 mM HEPES, 140 mM NaCl, 1mM EDTA, 0.09% NaN3 pH7.4
• Clonality: Monoclonal
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: Annexin V
• Application Info: This Annexin V preparation has been pre-titrated and quality-tested for flow cytometry using mouse thymocytes cultured overnight in the presence of dexamethasone to induce apoptosis. The Annexin V conjugate has been diluted for use at 5 uL per test, defined as the amount of protein that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e7 cells.
• Reference:

  Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH. 1994. Blood. 84(5):1415-1420.

  Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. 1995. J. Immunol. Methods. 184(1):39-51.