Ankyrin G Antibody (OASE00252)

Catalog No: OASE00252

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41. Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) EAAC1 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S106-20
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, ICC, IF
• Additional Information: Background Info: Detects over 200kDa.
  Scientific Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Ankyrin-G in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein 1000 C-terminal amino acids of human Ankyrin G encompassing all of Ankyrin G with the exception of Ankyrin repeats
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects >~200kDa.
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
• Description: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.

Target Info

• Gene Symbol: Ankyrin G
• Gene Full Name: ankyrin 3, node of Ranvier (ankyrin G)
• Alias Symbols: ANK-3, ANK3, ankyrin 3, Ankryin G119, brain specific ankyrin G, CHANK3, ankyrin 3 (G), Ankyrin-3, Ankyrin-G, node of Ranvier (ankyrin G), Ankyrin 3, node of Ranvier, Ankyrin G119, FLJ45464, OTTHUMP00000217458, OTTHUMP00000217575, RP11 369L1.1
• NCBI Gene Id: 288
• Uniprot ID: Q12955
• Protein Accession #: NP_066267.2