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ANKRD55 ELISA Kit (Mouse) (OKCA01628)

96 Wells
$610.00
In Stock
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Predicted Species Reactivity:
Mouse
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3.5 Hours
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat107103-110
1:28582-89
1:49894-102
1:810095-105
Kit Principle:
Aviva Systems Biology Ankrd55 ELISA Kit (Mouse) (OKCA01628) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ankrd55 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ankrd55 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Ankrd55 captured in the well.
Kit Range:
31.25 - 2,000 pg/mL
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)8683-89
EDTA Plasma (n=4)10298-106
Kit Component:
ComponentAmount
Ankrd55 Microplate96 Wells (12 x 8 Well strips)
Ankrd55 Lyophilized Standard2 x 2 ng
100X Biotinylated Ankrd55 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
7.8 pg/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Gene Symbol:
ANKRD55
Official Gene Full Name:
ankyrin repeat domain 55
Alias Symbols:
FLJ11795, MGC126013, MGC126014
NCBI Gene Id:
77318
Protein Name:
Ankyrin repeat domain-containing protein 55
Swissprot Id:
Q8BLD6
Protein Accession #:
NP_001161875.1
Nucleotide Accession #:
NM_001168403.1
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Ankrd55.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Ankrd55.

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