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Catalog No: OKCD04301
Size:96WELLS
Price: $675.00
SKU
OKCD04301
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Datasheets/ManualsPrintable datasheet for ANKRD12 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ankyrin Repeat Domain Protein 12 (ANKRD12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ankyrin Repeat Domain Protein 12 (ANKRD12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ankyrin Repeat Domain Protein 12 (ANKRD12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ankyrin Repeat Domain Protein 12 (ANKRD12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ankyrin Repeat Domain Protein 12 (ANKRD12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ankyrin Repeat Domain Protein 12 (ANKRD12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ANKRD12 Microplate96 Wells (12 x 8 Well strips)
ANKRD12 Lyophilized Standard2
100X ANKRD12 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.112 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolANKRD12
Alias SymbolsANCO1, GAC-1, ANCO-2, Nbla00144
NCBI Gene Id23253
Protein Nameankyrin repeat domain-containing protein 12
Description of TargetThis gene encodes a member of the ankyrin repeats-containing cofactor family. These proteins may inhibit the transcriptional activity of nuclear receptors through the recruitment of histone deacetylases. The encoded protein interacts with p160 coactivators and also represses transcription mediated by the coactivator alteration/deficiency in activation 3 (ADA3). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Uniprot IDQ6UB98
Protein Accession #NP_001077094.1
Nucleotide Accession #NM_001083625.2
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