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Catalog No: OCOA08246
Size:20UG
Price: $275.00
SKU
OCOA08246
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ANKHD1 Protein Lysate (OCOA08246)

Datasheets/ManualsPrintable datasheet for ANKHD1 Protein Lysate (OCOA08246)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionANKHD1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolANKHD1, ANKHD1-EIF4EBP3
Gene Full NameANKHD1-EIF4EBP3 readthrough, ankyrin repeat and KH domain containing 1
Alias SymbolsANKHD1-EIF4EBP3 protein;ankyrin repeat and KH domain-containing protein 1;HIV-1 Vpr-binding ankyrin repeat protein;MASK;MASK1;MASK-4E-BP3 alternate reading frame;MASK-BP3;MASK-BP3ARF;Multiple ankyrin repeats single KH domain;multiple ankyrin repeats, single KH-domain homolog;PP2500;VBARP.
NCBI Gene Id404734, 54882
Protein NameNM_024668, NP_078944, Q8IWZ3
Description of TargetAnkyrin repeat and KH domain-containing protein 1, ankyrin repeat and KH domain-containing protein 1 isoform 3 [Homo sapiens], Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8IWZ3
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_078944
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_024668
Molecular Weight64.9 kDa
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