ANKH Antibody - C-terminal region (OAAB06341)

Catalog No: OAAB06341

Size:400UL

Western blot analysis of ANKH Antibody (C-term) (Cat. #OAAB06341) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.

ANKH Antibody (C-term) (Cat. #OAAB06341) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ANKH Antibody (C-term) (Cat. #OAAB06341) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: Flow cytometry, Immunohistochemistry-Paraffin, Western blot
• Additional Information: Cellular Location: Membrane; Multi-pass membrane protein
  Tissue Location: Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
  Optimal dilutions should be determined by the end user.
• Other Applications Image 1 Data: ANKH Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Publications:

  Szeri, et al. The membrane protein ANKH is crucial for bone mechanical performance by mediating cellular export of citrate and ATP. PLoS Genetics 2020 Jul 8 16(7):e1008884. 32639996

• Description:
  View product citations for ANKH Antibody - C-terminal region (OAAB06341) on CiteAb

Target Info

• Gene Symbol: ANKH
• Gene Full Name: ANKH inorganic pyrophosphate transport regulator
• Alias Symbols: ANK;ankylosis, progressive homolog;CCAL2;CMDJ;CPPDD;Fn54 protein;HANK;MANK;progressive ankylosis protein homolog;SLC62A1.
• NCBI Gene Id: 56172
• Protein Name: NP_473368.1, P58366, Q9HCJ1, Q9JHZ2
• Description of Target: ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.
• Uniprot ID: https://www.uniprot.org/uniprot/?query=P58366+OR+Q9HCJ1+OR+Q9JHZ2
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_473368.1
• Molecular Weight: 54 kDa