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ANK1 Antibody - C-terminal region (ARP42566_T100)

100 ul
$249.00
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Conjugation Options

ARP42566_T100-FITC Conjugated

ARP42566_T100-HRP Conjugated

ARP42566_T100-Biotin Conjugated

Gene Symbol:
ANK1
Official Gene Full Name:
Ankyrin 1, erythrocytic
NCBI Gene Id:
286
Protein Name:
Ankyrin 1 isoform 5 variant EMBL BAD97298.1
Swissprot Id:
Q53ER1
Protein Accession #:
NP_065211
Nucleotide Accession #:
NM_020478
Alias Symbols:
ANK, SPH1, SPH2
Replacement Item:
This antibody may replace item sc-371924, HPA056953
Description of Target:
Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
Protein Size (# AA):
155
Molecular Weight:
18kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Protein A purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ANK1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ANK1.
Immunogen:
The immunogen is a synthetic peptide directed towards the C terminal region of human ANK1
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Tested Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Complete computational species homology data:
Anti-ANK1 (ARP42566_T100)
Peptide Sequence:
Synthetic peptide located within the following region: VVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
UBC; SPTA1; SUMO1; CD44; TGFBR1; SPTBN1; KCTD6; OBSCN; CUL3; NEDD8; SPTB; BLOC1S6; NFASC; NBPF3; TIAM1; L1CAM; TTN; SPTAN1; RHAG; EPB42; ITPR3; SLC4A1; SLC4A3;
Blocking Peptide:
For anti-ANK1 (ARP42566_T100) antibody is Catalog # AAP42566 (Previous Catalog # AAPP24804)
Datasheets/Manuals:
Printable datasheet for anti-ANK1 (ARP42566_T100) antibody
Target Reference:
Chang,T.L., (2004) Cell. Mol. Biol. (Noisy-le-grand) 50 (1), 59-66
Publications:

Toral-Ojeda, I; Aldanondo, G; Lasa-Elgarresta, J; Lasa-Fernández, H; Fernández-Torrón, R; López de Munain, A; Vallejo-Illarramendi, A; Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. 18, e7 (2016). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 27055500

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1 Item(s)

10/01/2018 03:17
  • Overall Experience:
  • Quality:
Mouse Brain Tissue in WB

Anonymously submitted through Aviva's "Free Antibody Vial for your Review!" promotion. 

ANK1

"We used this antibody for western blot of mouse tissue at a 1:500 dilution (see image attached) with excellent results."

1. Sample type/lane description: Mouse brain

Lane 1: Mouse brain tissue (quantity not given)

Lane 2: Mouse brain tissue (quantity not given)

2. Primary antibody dilution: 1:500

3. Secondary antibody and dilution: Licor goat anti-rabbit 790: 1:10,000.

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