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Catalog No: OPPA00514 (Formerly GWB-D91E44)
Size:2UG
Price: $75.00
SKU
OPPA00514
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ANGPTL4 Human - Recombinant Human Angiopoietin-like Protein 4 (OPPA00514)

Datasheets/ManualsPrintable datasheet for OPPA00514
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
Product FormatLyophilized 0.05M Acetate buffer (pH 4)
Physical Appearance: White freeze-dried powder
HostE. Coli
Reconstitution and StorageAdd 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited. Store lyophilized Angiopoietin-like Protein 4 Human recombinant at -20C. Aliquot the ANGPTL4 after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted ANGPTL4 can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C.
PurificationOne-step procedure using affinity Ni-NTA chromatography.
Concentration0.5 mg/ml (prior to lyophilization)
PurityGreater than 95% as determined by SDS-PAGE.
SpecificityThe amino acid sequence of the ANGPTL4 Human recombinant is 100% homologous to the 26-229 amino acid sequence of the Angiopoietin-like Protein-4 precursor without signal sequence.
Peptide SequenceMRGSHHHHHHGMASHMGPVQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSGLFEIQPQGSPPFLVNCKMTSDGGWTVIQR.
TagN-terminal His Tag
Gene SymbolANGPTL4
Alias SymbolsNL2, ARP4, FIAF, HARP, PGAR, HFARP, TGQTL, UNQ171, pp1158
NCBI Gene Id51129
Protein NameAngiopoietin-related protein 4
Description of TargetFIAF (fasting-induced adipose factor) a.k.a ANGPTL4 or PGAR or HFARP is an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. ANGPTL4 is found in human and mouse blood plasma both as a native protein and in a truncated form. In human white adipose tissue and SGBS adipocytes, only the native form of ANGPTL4 could be detected, whereas in mice the differentiation of mouse 3T3-L1 adipocytes is associated with the production of truncated ANGPTL4. However, the truncated ANGPTL4 is produced by human liver. In human blood plasma FIAF is mainly presented in a truncated form (FIAF-S2), whose levels treatment increases (as shown by experimental data). There is an inter individual variation in ANGPTL4 levels of both the truncated and the native form, however those levels were not influenced by prolonged semistarvation and are not associated with body mass index.
Uniprot IDQ9BY76
Protein Accession #NP_001034756.1
Protein Size (# AA)Recombinant
Molecular Weight25 kDa
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