Catalog No: OKBB00477
Size:96 Wells
Price: $583.00
SKU
OKBB00477
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human ANGPTL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL4 has been precoated onto 96-well plates. Standards(NS0, L165-S406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ANGPTL4 amount of sample captured in plate.
ELISA Kit Range625 pg/ml - 40,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)6.112.725.36.713.827.5
SD0.350.751.540.521.122.28
CV%5.75.96.17.88.18.3
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human ANGPTL4 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human ANGPTL4 Standard2 x 40 ng/tube
100X Biotinylated Anti-Human ANGPTL4 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<20 pg/ml
SpecificityNatural and recombinant human ANGPTL4
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: L165-S406
DescriptionFor quantitative detection of human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolANGPTL4
Gene Full Nameangiopoietin-like 4
Alias Symbols425O18 1, Angiopoietin like 4, Angiopoietin related protein 4, ANGPT L2, ANGPT L4, ANGPTL2, ANGPTL4, Fasting induced adipose factor, FIAF, Fibrinogen/angiopoietin related protein, Hepatic fibrinogen/angiopoietin related protein, HFARP, Major histocompatibility complex region NG27, NL2, Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein, PGAR, pp1158, PPARG angiopoietin related protein, PSEC0166, Secreted protein Bk89, Weakly similar to angiopoietin 1
NCBI Gene Id51129
Protein NameAngiopoietin-related protein 4
Description of TargetAngiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.
Uniprot IDQ9BY76
Protein Accession #NP_001034756.1
Nucleotide Accession #NM_001039667.2
Write Your Own Review
You're reviewing:ANGPTL4 ELISA Kit (Human) (OKBB00477)
Your Rating
We found other products you might like!