ANGPTL2 ELISA Kit (Human) (OKBB01406)

Catalog No: OKBB01406

Size:96 wells

ANGPTL2 ELISA Kit (Human) (OKBB01406) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human ANGPTL2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL2 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: Q23-H493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ANGPTL2 amount of sample captured in plate.
• ELISA Kit Range: 312 pg/ml - 20,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	590	1670	7933	594	1958	8471
  Standard deviation	30.68	71.81	325.25	39.20	113.56	559.09
  CV(%)	5.2	4.3	4.1	6.6	5.8	6.6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human ANGPTL2 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human ANGPTL2 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human ANGPTL2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	590	572	610	690	615	45.06	7.3%
  Sample 2	1670	1703	1941	1771	1771	104.55	5.9%
  Sample 3	7933	8323	9098	7778	8283	510.72	6.1%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
  O.D.	0.068	0.110	0.152	0.228	0.376	0.756	1.179	2.026

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <50 pg/ml
• Specificity: Natural and recombinant human ANGPTL2
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q23-H493
• Description: For quantitative detection of human ANGPTL2 in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: ANGPTL2
• Gene Full Name: angiopoietin like 2
• Alias Symbols: Angiopoietin-related protein 2; Angiopoietin-like protein 2
• NCBI Gene Id: 23452
• Protein Name: Angiopoietin-related protein 2
• Description of Target: Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
• Uniprot ID: Q9UKU9
• Protein Accession #: NP_036230.1
• Nucleotide Accession #: NM_012098.2