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Tested Species Reactivity | Human |
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Product Format | Lyophilized The protein is deposited as a clear and colorless film that is not always visible. Even if the vial appears empty, reconstitute according to instructions below and use as intended. |
Application | CA |
Additional Information | Endotoxin Level: Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
Reconstitution and Storage | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to one week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (ex. 0.1% BSA) and store in working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles. Expires 1 year from date of receipt. |
Purity | 95% |
Biological Activity | Using a concentration of 0.2 ug/mL, a tubulogenesis assay with human umbilical vein endothelial cells (HUVECs) is performed. |
Protein Sequence | DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YT |
Source | CHO cells |
Tag | C-terminal His tag |
Reference | Masonpierre PC, Suri C, Jones PF, Bartunkova S, Wiegand SJ, Radziejeski C, Compton D, McClain J, Aldrich TH et al. 1997. Science. 277(5322): 55-60. Murdoch C, Tazzyman S, Webster S adn Lewis CE. 2007. J Immunol. 178(11): 7405-7411. Tsigkos S, Koutsilieris M and Papapetropoulos A. 2003. Expert Opin Investig Drugs. 12(6): 933-941. Sato A, Iwama A, Takakura N, Nishio H, Yancopoulos GD and Suda T. 1998. Int Immunol. 10(8):1217-1227. Singh H, Tahir TA, Alawo DO, Issa E and Brindle NP. 2011. Biochem Soc Trans. 39(6): 1592-1596. Yuan HT, Khankin EV, Karumanchi SA and Parikh SM. 2009. Mol Cell Biol. 29(8): 2011-2022. |
Gene Symbol | ANGPT2 |
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Gene Full Name | angiopoietin 2 |
Alias Symbols | ANG2, AGPT2 |
NCBI Gene Id | 285 |
Protein Name | angiopoietin-2 |
Description of Target | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. |
Molecular Weight | 50.1 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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