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ANGPT2 ELISA Kit (Rat) (OKDD00624)

Catalog#: OKDD00624
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
This product is Genway GWB-KBBOW6
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~4 Hours
Kit Principle Aviva Systems Biology ANGPT2 ELISA Kit (Rat) (OKDD00624) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ANGPT2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANGPT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ANGPT2 captured in well.
Kit Range 0.156-10 ng/mL
Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

Kit Component
Anti-ANGPT2 Microplate96 Wells (12 x 8 Well Strips)
ANGPT2 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ANGPT2 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma
Sensitivity <0.054 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of ANGPT2. No significant cross-reactivity or interference between ANGPT2 and analogues was observed.
Assay Info Quantitative Sandwich ELISA
Gene Symbol ANGPT2
Official Gene Full Name angiopoietin 2
Alias Symbols Agpt2, Ang-2, ANG-2, Angiopoietin-2
NCBI Gene Id 89805
Protein Name angiopoietin-2
Description of Target Binds to tek/tie2, competing for the angpt1 binding site, and modulating angpt1 signaling. can induce tyrosine phosphorylation of tek/tie2 in the absence of angpt1. in the absence of angiogenic inducers, such as vegf, angpt2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. in concert with vegf, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (by similarity).
Swissprot Id O35462
Protein Accession # NP_604449.1
Nucleotide Accession # NM_134454.1
Protein Size (# AA) 496
Molecular Weight 57 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ANGPT2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ANGPT2.
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