ANGPT1 Recombinant Protein (Human) (OPED00034)

Catalog No: OPED00034

Size:10 ug

Figure 1: COMP (rat):Angiopoietin-1 (human) (rec.) forms mainly pentameric structures. Method: Glycerol spraying/low angle rotary metal-shadowed specimens were imaged by transmission electron microscopy.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human|Rat|Rattus norvegicus
• Product Format: Liquid PBS containing 0.01% CHAPS
• Host: CHO cells
• Additional Information: FLAG is a registered trademark of Sigma-Aldrich Co.
• Reconstitution and Storage: Store long-term at -80°C. After opening, prepare aliquots and store at -80°C. Working aliquots are stable for up to 3 months when stored at -80°C. Avoid repeated freeze/thaw cycles.
• Immunogen: The fibrinogen-like domain (aa284-498) of human Ang-1 is fused at the N-terminus to the coiled-coil domain of rat COMP and to a FLAG-tag.
• Concentration: Varies by lot. See vial for concentration.
• Purity: >90% (SDS-PAGE)
• Biological Activity: Induces phosphorylation of Tie-2 in primary cultured human and mouse vascular endothelial cells. More potent than native Ang-1.

Target Info

• Reference: Angiopoietin-1 promotes LYVE-1-positive lymphatic vessel formation: T. Morisada, et al.; Blood 105, 4649 (2005), Abstract;
  Multiple angiopoietin recombinant proteins activate the Tie1 receptor tyrosine kinase and promote its interaction with Tie2: P. Saharinen, et al.; J. Cell Biol. 169, 239 (2005), Abstract;
  COMP-Ang1: a designed angiopoietin-1 variant with nonleaky angiogenic activity: C.H. Cho, et al.; PNAS 101, 5547 (2004), Abstract; Full Text
  Designed angiopoietin-1 variant, COMP-Ang1, protects against radiation-induced endothelial cell apoptosis: C.H. Cho, et al.; PNAS 101, 5553 (2004), Abstract; Full Text
• Gene Symbol: ANGPT1|Comp
• Gene Full Name: angiopoietin 1|cartilage oligomeric matrix protein
• Alias Symbols: AGP1;AGPT;ANG1;ANG-1;angiopoietin-1;cartilage oligomeric matrix protein;HAE5.
• NCBI Gene Id: 25304|284
• Protein Name: Angiopoietin-1|Cartilage oligomeric matrix protein
• Description of Target: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.|May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (By similarity). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
• Uniprot ID: P35444|Q15389
• Protein Accession #: NP_001287843.1
• Nucleotide Accession #: NM_001300914.1
• Protein Size (# AA): Recombinant