ANGPT1 ELISA Kit (Dog) (OKEH07217)

Catalog No: OKEH07217

Size:96T

Dog ANGPT1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	99-99% (n = 20)	100-110% (n = 20)	101-101% (n = 20)	104-115% (n = 20)
  EDTA plasma(n=5)	98-107% (n = 20)	101-114% (n = 20)	101-113% (n = 20)	91-103% (n = 20)
  heparin plasma(n=5)	89-99% (n = 20)	111-120% (n = 20)	88-98% (n = 20)	106-118% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ANGPT1 ELISA Kit (Dog) (OKEH07217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANGPT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANGPT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANGPT1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.6% (n = 20)
  Mean Inter-assay CV%: <=9.7% (n = 20)
• ELISA Kit Component:
  • ANGPT1 Microplate: 96 Wells (12 x 8 Well strips)
  • ANGPT1 Lyophilized Standard: 2
  • 100X Biotinylated ANGPT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  Subcellular Location: Secreted
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Dog Angiopoietin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANGPT1
• Gene Full Name: angiopoietin 1
• Alias Symbols: ANG1;ANG-1;angiopoietin-1.
• NCBI Gene Id: 403656
• Protein Name: Q60FC1
• Description of Target: Angiopoietin-1
• Uniprot ID: https://www.uniprot.org/uniprot/Q60FC1
• Protein Accession #: NP_001005754.1
• Nucleotide Accession #: NM_001005754.2