Size:96 Wells
$619.00
In Stock
SKU
OKEH02621
Request Bulk
Order Quote
Aviva's
Satisfaction
Guarantee
Contact Us:
  • Toll Free: (888)880-0001
  • Phone: (858)552-6979
  • Email: info@avivasysbio.com
Shipping Info:
  • $40: Antibody & Protein in US
  • $50: 1-2 Kits in US
  • Contact us for international orders.

Angiotensin I ELISA Kit (OKEH02621)

Catalog#: OKEH02621
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species Reactivity All
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology Angiotensin I ELISA Kit (OKEH02621) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Angiotensin I antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Angiotensin I and incubated. The Angiotensin I found in the sample or standards competes with the biotinylated Angiotensin I for limited binding sites on the immobilized anti-Angiotensin I antibody. Excess unbound biotinylated Angiotensin I and sample or standard Angiotensin I is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Angiotensin I captured in the well and inversely proportional to the amount of Angiotensin I which was contained in the sample or standard.
Kit Range 78 - 5000pg/mL
Kit Recovery Mean recovery when spiking into Serum and Plasma = 103%
Kit Reproducibility Mean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
Kit Component
ComponentAmount
Anti-Angiotensin I Microplate96 Wells (12 x 8 Well strips)
Angiotensin I Lyophilized Standard2 x 5,000 pg
Sample Diluent1 x 20 mL
100X Angiotensin I-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional Information Target Abbr: Ang-I
Target Name: Angiotensin I
:: Pubchem: 3081372
:: Chemical formula: C62H89N17O14
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity 22 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant General Angiotensin I
Assay Info Assay Methodology: Quantitative Competitive ELISA
Alias Symbols Ang-I, Angiotensin I, Ang-I
Protein Name Angiotensin I
Description of Target Angiotensins are small peptides derived from angiotensinogen. Several of the known Angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I has no known effector function but it is an immediate precursor of Angiotensin II. Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Arg‐vasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A and Angiotensin III, also bind AT1 receptors. Angiotensin (1‐7) binds a different receptor called MAS‐1 which has opposite effects (vasodilation, natriuresis, antiproliferation, NO release, PGE release, and apoptosis). Angiotensin IV binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimer’s disease. The peptide LVV‐hemorphin‐7, which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that Discover more at www.abcam.com 3 INTRODUCTION have been identified, including Ang (1‐9), Ang (1‐12), Ang V(3‐7), as well as several other shorter peptides that have undetermined functions. Angiotensins can be present in very low concentrations in some biological samples. In such cases, dilution of samples to avoid “non‐specific” interference by any present factors is not productive because the angiotensin analyte is also diluted to levels far below the minimum detection concentration. Thus, investigators have used several procedures for extracting Angiotensins from biological samples prior to using them for immunoassays. Angiotensins share common sequences, and in some cases they cannot be discriminated by immunoassays. Prior extraction and separation by HPLC may, thus, be required.
Write Your Own Review
You're reviewing:Angiotensin I ELISA Kit (OKEH02621)
Your Rating