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Catalog No: OKCD02360
Size:96WELLS
Price: $650.00
SKU
OKCD02360
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Datasheets/ManualsPrintable datasheet for AMY2A5 ELISA Kit (Mouse) (OKCD02360)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-90%99-105%89-98%87-95%
EDTA Plasma (n=5)85-94%81-102%95-103%84-92%
Heparin Plasma (n=5)81-96%88-104%80-96%92-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase, Alpha 2A (AMY2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase, Alpha 2A (AMY2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase, Alpha 2A (AMY2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amylase, Alpha 2A (AMY2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)81-8985
EDTA Plasma (n=5)82-10196
Heparin Plasma(n=5)97-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amylase, Alpha 2A (AMY2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amylase, Alpha 2A (AMY2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-AMY2A5 Microplate96 Wells (12 x 8 Well strips)
AMY2A5 Lyophilized Standard2
100X Biotinylated AMY2A5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity1.39 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Amylase Alpha 2, Pancreatic (AMY2). No significant cross-reactivity or interference between Amylase Alpha 2, Pancreatic (AMY2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAmy2a2|Amy2a3|Amy2a4|Amy2a5
Gene Full Nameamylase 2a2|amylase 2a3|amylase 2a4|amylase 2a5
Alias Symbols1,4-alpha-D-glucan glucanohydrolase;1810008N23Rik;Amy;Amy-;Amy2;Amy-2;Amy2a;amylase 2, pancreatic;amylase 2a2;amylase 2a2, pancreatic;amylase 2a3;amylase 2a3, pancreatic;amylase 2a4;amylase 2a4, pancreatic;amylase 2a5, pancreatic;mAmy2-;mAmy2-2;mAmy2-3;mAmy2-4;mAmy2-5;PA;Pancreatic alpha-amylase.
NCBI Gene Id100043684|100043686|100043688|109959
Protein NamePancreatic alpha-amylase
Description of TargetPancreatic alpha-amylase
Uniprot IDP00688
Protein Accession #NP_001036176.1
Nucleotide Accession #NM_001042711.2
Protein Size (# AA)508
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