AMY2A ELISA Kit (Human) (OKCD07408)

Catalog No: OKCD07408

Size:96WELLS

AMY2A ELISA Kit (Human) (OKCD07408) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase, Alpha 2A (AMY2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase, Alpha 2A (AMY2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase, Alpha 2A (AMY2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amylase, Alpha 2A (AMY2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amylase, Alpha 2A (AMY2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amylase, Alpha 2A (AMY2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AMY2A Microplate: 96 Wells (12 x 8 Well strips)
  • AMY2A Lyophilized Standard: 2
  • 100X AMY2A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.255ng/mL

Target Info

• Gene Symbol: AMY2A
• Gene Full Name: amylase alpha 2A
• Alias Symbols: 1,4-alpha-D-glucan glucanohydrolase;AMY2;amylase alpha 2A (pancreatic);glycogenase;PA;pancreatic alpha-amylase;pancreatic amylase alpha 2A.
• NCBI Gene Id: 279
• Protein Name: Pancreatic alpha-amylase
• Description of Target: Pancreatic alpha-amylase
• Uniprot ID: P04746
• Protein Accession #: NP_000690.1
• Nucleotide Accession #: NM_000699.3