Catalog No: OKEH00326
Size:96T
Price: $600.00
SKU
OKEH00326
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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AMSH ELISA Kit (Human) : 96 Wells (OKEH00326)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AMSH ELISA Kit (Human) (OKEH00326) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AMSH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for AMSH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AMSH captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-Assay Precision - < 4.2%, n = 20
Mean Inter-Assay Precision - < 7.6%, n = 20
ELISA Kit Component
ComponentAmount
AMSH Microplate96 Wells (12 x 8 Well strips)
AMSH Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated AMSH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.6 ng/mL
SpecificityNatural and recombinant Human alpha-MSH
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAMSH
Alias SymbolsAMSH, MICCAP
NCBI Gene Id10617
Protein NameSTAM-binding protein
Description of TargetIt is the most important of the melanocyte-stimulating hormones (MSHs; also known as melanotropins) in stimulating melanogenesis, a process which in mammals (including humans) is responsible for pigmentation primarily of the hair and skin. It also plays a role in feeding behavior, energy homeostasis, and sexual activity. It is generated as a proteolyic cleavage product from ACTH (1-13), which is in turn a cleavage product of proopiomelanocortin (POMC).
Uniprot IDO95630
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