Catalog No: OKCD04304
Size:96WELLS
Price: $675.00
SKU
OKCD04304
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Datasheets/ManualsPrintable datasheet for AMR ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenomedullin Receptor (AMR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenomedullin Receptor (AMR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenomedullin Receptor (AMR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adrenomedullin Receptor (AMR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin Receptor (AMR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin Receptor (AMR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AMR Microplate96 Wells (12 x 8 Well strips)
AMR Lyophilized Standard2
100X AMR HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity0.054 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAMR
Alias SymbolsAMR, 7TMR, ADMR, AM-R, G10D, L1-R, gamrh, hrhAMR
NCBI Gene Id11318
Protein NameG-protein coupled receptor 182
Description of TargetAdrenomedullin is a potent vasodilator peptide that exerts major effects on cardiovascular function. This gene encodes a seven-transmembrane protein that belongs to the family 1 of G-protein coupled receptors. Studies of the rat counterpart suggest that the encoded protein may function as a receptor for adrenomedullin.
Uniprot IDO15218
Protein Accession #NP_009195.1
Nucleotide Accession #NM_007264.3
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