Catalog No: OPCA02037
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OPCA02037
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Datasheets/ManualsPrintable datasheet for ampC Recombinant Protein (Pseudomonas aeruginosa) (OPCA02037)
Product Info
Predicted Species ReactivityPseudomonas aeruginosa
Product FormatLiquid or Lyophilized powder
HostPseudomonas aeruginosa
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Formulation20 mM Tris-HCl based buffer, pH 8.0
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequenceGEAPADRLKALVDAAVQPVMKANDIPGLAVAISLKGEPHYFSYGLASKEDGRRVTPETLFEIGSVSKTFTATLAGYALTQDKMRLDDRASQHWPALQGSRFDGISLLDLATYTAGGLPLQFPDSVQKDQAQIRDYYRQWQPTYAPGSQRLYSNPSIGLFGYLAARSLGQPFERLMEQQVFPALGLEQTHLDVPEAALAQYAQGYGKDDRPLRVGPGPLDAEGYGVKTSAADLLRFVDANLHPERLDRPWAQALDATHRGYYKVGDMTQGLGWEAYDWPISLKRLQAGNSTPMALQPHRIARLPAPQALEGQRLLNKTGSTNGFGAYVAFVPGRDLGLVILANRNYPNAERVKIAYAILSGLEQQGKVPLKR
Storage BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
SourceE. Coli
Protein Range27-397 aa
TagN-terminal 6xHis-SUMO-tagged
ReferenceCloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase.Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W.Biochem. J. 272:627-631(1990)
Gene SymbolAMPC
Alias SymbolsCephalosporinase
NCBI Gene Id878149
Swissprot IdP24735
Protein Accession #NP_252799.1
Nucleotide Accession #NC_002516.2
Protein Size (# AA)27-397 aa
Molecular Weight56.7 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ampC.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ampC.
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