AMOT Antibody - N-terminal region (ARP34660_P050)

Catalog No: ARP34660_P050

WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human heart

Host: Rabbit
Target Name: AMOT
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 1ug/ml

Rabbit Anti-AMOT Antibody
Catalog Number: ARP34660_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Membrane(tight junctions - intercalated disks)
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AMOT
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77%
• Peptide Sequence: Synthetic peptide located within the following region: TANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELATARSTNE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-AMOT (ARP34660_P050) antibody is Catalog # AAP34660 (Previous Catalog # AAPP05850)
• Reference: Holmgren,L., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (24), 9208-9213
• Publications:

  VCAM-1 induces signals that stimulate ZO-1 serine phosphorylation and reduces ZO-1 localization at lung endothelial cell junctions. J Leukoc Biol. 104, 215-228 (2018). 29889984

Target Info

• Gene Symbol: AMOT
• Gene Full Name: Angiomotin
• Alias Symbols: KIAA1071
• NCBI Gene Id: 154796
• Protein Name: Angiomotin
• Description of Target: AMOT belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Uniprot ID: Q4VCS5
• Protein Accession #: NP_573572
• Nucleotide Accession #: NM_133265
• Protein Size (# AA): 675
• Molecular Weight: 73kDa
• Protein Interactions: AMOT; C1orf216; WDYHV1; MED4; SNAPC5; PPP2R4; LURAP1; UNC119; LMO4; PIN1; MAGEA4; KRT31; KRT15; FUS; SEPT1; CEP76; HAUS2; LATS2; CEP250; CDKN1A; ACTB; ACTA1; YAP1; SART3; AURKB; MAPK3; BAG3; WWOX; ITCH; LATS1; YWHAG; UBC; PARK2; AMOTL1; SPICE1; MPP7; CNTR