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Datasheets/Manuals | Printable datasheet for OASE00286-FITC |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Monoclonal |
Clone | S86-36 |
Isotype | IgG1 |
Host | Mouse |
Conjugation | FITC: Fluorescein Isothiocyanate |
Application | WB, ICC, IHC |
Additional Information | Background Info: Detects 60-80kDa depending on maturity/glycosylation |
:: | Scientific Background: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction. |
:: | Certificate of Analysis: 1ug/mL was sufficient for detection of AMIGO-1 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1, accession number Q86WK6 |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Dilution | 1:1000 WB |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973. 2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270. 3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896. 4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274. |
Gene Symbol | AMIGO1 |
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Gene Full Name | adhesion molecule with Ig-like domain 1 |
Alias Symbols | ALI2, AMIGO, AMIGO-1 |
NCBI Gene Id | 57463 |
Uniprot ID | Q86WK6 |
Protein Accession # | NP_065754.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "AMIGO-1 Antibody : FITC (OASE00286-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
This target may also be called "ALI2, AMIGO, AMIGO-1" in publications.
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What is the shipping cost for "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "AMIGO-1 Antibody : FITC (OASE00286-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AMIGO1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AMIGO1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AMIGO1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AMIGO1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AMIGO1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AMIGO1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.