AMFR Antibody - N-terminal region (OAAB15786)

Catalog No: OAAB15786

Size:400ul

Western blot analysis of AMFR (arrow) using AMFR Antibody (N-term) (Cat.# OAAB15786). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).

AMFR is supported by BioGPS gene expression data to be expressed in HEK293

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term) (Cat.# OAAB15786), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using AMFR Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Precautions: AMFR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor
  Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-601 amino acids of human AMFR.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  AMFR is supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: AMFR
• Alias Symbols: GP78, RNF45
• NCBI Gene Id: 267
• Protein Name: E3 ubiquitin-protein ligase AMFR
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9UKV5
• Protein Accession #: NP_001135.3
• Molecular Weight: 72996 Da